Search results for "Derivative spectroscopy"
showing 2 items of 2 documents
Influence of the differentiation system on the analytical parameters for the spectrophotometric determination of clonazepam in urine
1991
Abstract Electronic and mathematical differentiation of the ultraviolet visible spectra were compared from the study of the analytical parameters of the direct determination of clonazepam in urine samples. Third-order derivative spectra were used. The mathematical differentiation provides higher sensitivity, a lower limit of detection, and better accuracy and precision than the electronic one. The use of a diode array instrument provides lower sensitivity than that found using a conventional spectrometer; however, the limit of detection obtained in both cases is of the same order.
Computerized Interpretation of H P L C Chromatogramms by Means of Absorbance Ratio Method and Derivative Spectroscopy
1985
As in other chromatographic processes it is the aim of high pressure liquid chromatography (HPLC) to identify the components of a mixture, whereby there may be some information on the class of substances in question. The analytical procedure can shortly be desribed as follows. The mixture is injected in a mobile phase (solvent) and passes a column where the separation takes place. The separated components leave the column at different retention times. A following detection device generates a signal as a function of concentration (chromatographic peak). Usually the components are identified by relating retention times of external standards to the times of occurence of chromatographic peaks. …